Recent publications have reported methods using 19and 24variable windows implemented on q exactive and q exactive hf at a resolution of 30,000 7,8, and 70 windows on q exactive hfx with fixed. Thermo fisher scientific exactive operating manual vii identifying safety information the exactive operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. Scope of this guide the q exactive hf software manual includes the following chapters. Exactive gc orbitrap gcms and q exactive gc orbitrap gcms.
Thermo scientific tsq 8000 evo user guide ix p preface this guide contains detailed information about how to use your thermo scientific tsq 8000 evo triplequadrupole gcms system. Thermo scientific exactive series operating manual pn bre0012255, revision a vii technical data for exactive series systems the table summarizes the most import ant technical data of the exactive. This q exactive instrument features high ion currents because of an slens, and fast highenergy collisioninduced dissociation peptide fragmentation because of parallel filling and detection modes. Thanks to its orbitrap mass analyzer, the exactive plus system delivers highresolution, accuratemass hram fullscan ms for fast, precise and. Thermo scientific exactive series operating manual pn bre0012255, revision a vii technical data for exactive series systems the table summarizes the most import ant technical data of the exactive series systems. Resources for optimal performance of your orbitrap fusion series mass spectrometer. Q exactive gc orbitrap gcmsms thermo fisher scientific. In addition, metworks software allows highresolution, accuratemass data from orbitrap, ltq ft, q exactive and exactive instruments to be fully leveraged. The q exactive hf system combines a stateoftheart segmented quadrupole for highperformance precursor ion selection with a highresolution, accuratemass hram ultrahighfield orbitrap mass analyzer to deliver a superior combination of scan speed, resolving power, mass accuracy, spectral quality and sensitivity. The image current from the detector is processed by an enhanced fourier transformation algorithm, doubling mass spectrometric resolution. It is specific to the thermo scientific q exactive mass spectrometer and will include instruction for electrospray ionization esi of proteins and peptides. Exactive series operating manual revision a thermo fisher. The results illustrate the benefits of the integration of ce and esi in a single dynamic process coupled with high resolution mass spectrometry. For information about performing these tasks, refer to the exactive series operating manual, the qexactivehf software manual or the q exactive hf tune help, and the ion max and ion maxs api source hardware manual.
Tune file on the desktop, or go to start all programs thermo exactive series tune. The thermo q exactive orbitrap is a high resolution mass spectrometer suitable for accurate mass measurement as well as msms studies. Resources for optimal performance of your orbitrap elite mass spectrometer. The stateoftheart peptide mapping data possible with the thermo scientific q exactive biopharma platform is a culmination of decades of leadership in the development of data dependent acquisition dda combined with the unmatched accuracy and resolution achieved by the thermo scientific orbitrap mass analyzer.
We use it in the lab to perform shotgun proteomic and metaproteomic analysis of biological specimens within various studies. I understand that i may be liable for a field service representatives travel or lodging expenses if they are una ble to carry out the installation on the pr escheduled date due to insufficient lab p reparation. Faster, more powerful speed productivity confidence the thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer features an ultrahighfield orbitrap analyzer which doubles its speed and resolution more samples run and amazing data quality. It also describes the modes of operation and principle hardware components of your exactive plus instrument. About this manual this exactive plus operating manual contains precautionary statements that can prevent personal injury, instrument damage, and loss of data if properly followed. Thermo scientific q exactive plus orbitrap lcmsms system. Thermo scientific q exactive orbitrap lcmsms system from. Exactive plus operating manual pn 23060, revision a i. Ltq orbitrap velos hardware manual brown university.
Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer. An instrument with attached lifting devices does not fit through a door. This course is designed for users that have previous lcms experience and are interested in protein and peptide analysis. A more detailed and thorough account of these procedures can be found in the qexactive operating manual. Operating manual for instructions about using the lifting devices.
Q exactive gc operating manual pn 1r1207060002, revision a i. Peptides, both large and small 3 65 amino acids, were identified, separated, and quantified. To view product manuals go to start programs thermo exactive manuals. Send us an email if you are looking for a specific resource or advice on how you can get the most out of your instrument. There are several ways to contact thermo fisher scientific. Please note that this guide does not contain any safe ty notices and precautionary statements. We know you, or someone you know, worked very hard to get your orbitrapbased mass spectrometer in your lab and even harder preparing your precious samples.
Based on an innovative combination of a hexabore capillary and ionfunnel based entrance optics which allow maximum capture of your analytes, this is the ideal qtof lcms for your proteomics research. Rapid level 3 characterization of biologics using a cesi 8000. Manuals for the delivered ion sources and other software. Q exactive hybrid quadrupole orbitrap mass spectrometer. Exactive operating manual manuals for the delivered ion sources and other software. Sciex, coupled to a thermo qexactive mass spectrometer was used for the analyses.
The tsq 8000 evo system provides the selectivity and sensitivity of a triplequadrupole gcms while also functioning as a highperformance single quadrupole. Page 112 the distance across a peak measured at a selected peakheight level, in minutes or mass units. Thus, it is mandatory to read also the q exactive operating manual and the ion max and ion maxs api source hardware manual. This q exactive hf software manual is intended for all personnel that need to perform measurements with the q exactive hf mass spectrometer, especially the key operator. The superior quality of q exactive plus msms data enables identification and quantitation of more compounds with greater confidence. Q exactive hybrid quadrupoleorbitrap mass spectrometer support. Recent publications have reported methods using 19and 24variable windows implemented on qexactive and qexactive hf at a resolution of 30,000 7,8, and 70 windows on q. Utilization of high resolution lcms for screening and quantitative analysis of pesticides in food matrix using a q exactive bench top orbitrap platform. To download user documentation from the thermo scientific.
Metworks software is compatible with all thermo scienti. Performance evaluation of the q exactive hfx for shotgun. Thermo orbitrap fusion series hardware manual pdf download. The defining difference of the q exactive compared with the exactive is the presence of a mass selective quadrupole analyzer between the ion source and the ctrap fig. You can access pdf files of the documents listed above from the data system computer. In order to generate a user defined mass calibration list, the current user role setting in the exactives instrument status window must be selected as advanced. It is capable of ionization using conventional heated electrospray hesi, atmospheric pressure chemical ionization. This guide is intended for all personnel who operate the q exactive plus mass spectrometer.
The q exactive is an excellent system that generates reproducible, solid datasets. See the respective chapters of the manual for details and. Jun 03, 2011 the q exactive also employs a 90 bent ion path from the source toward the mass analyzer in common with the exactive and in contrast to the ltq orbitrap instruments. The data system computer with the q exactive hf tune software is switched on and the mass spectrometer is placed in standby mode. The thermo scientific q exactive gc hybrid quadrupole orbitrap mass spectrometer provides an unmatched combination of sensitivity, massaccuracy and resolving power, helping answer todays most challenging analytical questions. The thermo scientific exactive plus orbitrap ms is a benchtop lcms system designed for highperformance, highthroughput screening, compound identification, and quantitative analysis. Thermo scientific q exactive mass spectrometer series calibration and maintenance. With bestinclass performance characteristics for both quantitative and discovery workflows, the q exactive.
Log in your mass spec qexactive session on the cores. Used thermo q exactive mass spectrometer for sale dotmed. The q exactive hfx ms takes identification and quantitation. It is capable of ionization using conventional heated electrospray hesi, atmospheric pressure chemical ionization apci, and nanoflow electrospray nanoesi.
Scope of this guide the exactive software manual includes the following chapters. For information about performing these tasks, see the exactive operating manual, the exactive software manual, or the exactive tune online help, and the ion max and ion maxs api source hardware manual. Isq 7000 user guide isq 7000 hardware manual isq 7000 spare parts guide isq series, tsq series and q exactive gc direct probe user guide tsq 9000 triple quadrupole ms 5 tsq 9000 preinstallation requirements guide tsq 9000 user guide tsq 9000 hardware manual tsq 9000 spare parts guide isq series, tsq series and q exactive gc direct probe user guide. The computer is not recognizing the mass spec thermo scientific q exactive. The thermo scientific tm q exactive tm hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation tm. Warning symbols alert the user to check for hazardous conditions. The user will need to create a user defined mass calibration list in order to calibration the exactive using the custom calibration solution outlined in section 1.
Thermo scientific user manuals download manualslib. If you have a q exactive gc system, go to start all programs thermo instruments manuals. See the respective chapters of the manual for details and additional instrument properties. Thermo q exactive the exactive plus orbitrap lcms is a benchtop full scan mass spectrometer designed for compound identification and highthroughput, highperformance screening. Exactive quickstart guide thermo fisher scientific. This guide is intended for all personnel who operate the q exactive mass spectrometer. Thermo scientific q exactive hf orbitrap lcmsms system. Mass spectrometers department of chemistry mellon college of. Demonstrates how to remove and clean the slens on the q exactive instrument series removing and cleaning the slens on thermo scientific q exactive mass spectrometers on vimeo join. Mass spectrometrybased proteomics using q exactive, a high. It is an easytouse, dedicated benchtop gcms system that provides the highest confidence in compound discovery, identification and quantitation for a comprehensive understanding of your samples. The peakheight level is usually specified as a percentage of the maximum peak height. Upgrade your maps peptide mapping thermo scientific.
Thermo fisher scientific is pleased to announce the release of thermo scientific watson lims 7. Thermo scientific q exactive plus orbitrap liquid chromatographyhigh resolution mass spectrometry lchrms system. With a fast scan speed and multiplexing capabilities, the q exactive plus mass spectrometer is an outstanding detector for fast chromatography separation techniques. Pulsed q collision induced dissociation pqd enables trapping of low mass. Exactive plus operating manual planet orbitrap mafiadoc. Thermo scientific q exactive orbitrap lcmsms system. What you need to know about calibration and maintenance of the thermo scientificq exactive mass spectrometer series. Tsq 8000 evo, and q exactive gc direct probe system user guide, pn 1r1205050006 to view product manuals if you have an isq lt or a tsq 8000 evo system, open the manuals folder on your desktop. Thermo scientific q exactive hf orbitrap lcmsms system higherquality data, faster than ever the thermo scientific q exactive hf benchtop lcmsms combines a highresolution accuratemass hram ultrahighfield orbitrap analyzer and a high performance quadrupole precursor ion selection to deliver unsurpassed speed while. It is simple to use and maintenance on the q exactive is usually straightforward when outlined in the manual. It is simple to use and maintenance on the q exactive is. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive. This q exactive plus quickstart guide gives an introduction on setting up and using the thermo scientific q exactive plus mass spectrometer. Q exactive hybrid quadrupoleorbitrap ms with zipchip system a comprehensive ceesims solution for biopharma applications.
Exactive plus orbitrap mass spectrometer thermo scientific. This manual should be kept near the instrument to be available for quick reference. This text is a guide to operating the qexactive mass spectroscopy. Thermo scientific content manual and documents official. Mass spectrometrybased proteomics using q exactive, a. Today, the q exactive gc system makes this comprehensive analysis a reality. Removing and cleaning the slens on thermo scientific q.
The thermo qexactive orbitrap is a high resolution mass spectrometer suitable for accurate mass measurement as well as msms studies. Exactive operating manual thermo fisher scientific. Thermo scientific orbitrap fusion series hardware manual. Q exactive resolution at mz 200 free injection time ms approx. Q exactive hf software manual thermo fisher scientific. In addition to this manual, thermo fisher scientific provides other documents for the exactive plus mass spectrometer that. This exactive software manual is intended for all personnel that need to perform measurements with the exactive mass spectrometer, especially the key operator. Tmt workflow on the q exactive series instrument parameter optimization and data analysis in proteome discoverer 2. All other trademarks are the property of thermo fisher scientific inc.
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